Training on preparing high-quality plasmid DNA

In order for the precise measurement of PERV gene copy number, it is definitely important to draw the accurate standard curve. In so doing, a high-quality plasmid DNA for each PERV genes is required.

Mr. Toan (NIAS-V) is taught how to go through the procedure by Drs Taniguchi & Arakawa (NILGS) during 26-27 Feb 2018.

 

Dr. Taniguchi point out a good colony of the competent cells identified to have the plasmid which is aimed to be used.

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Drs Taniguchi & Arakawa show the procedure of cell spreading on the agar medium plate.

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The plasmid DNA containing competent cells (E.Coli) is being spread on agar medium plates and going to be used for further PERV gene copy number analysis.

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