Short-term Study in Japan about Vitrification Procedures for Embryos, 20 Feb – 18 March, 2017

One of the outputs of the Project is to “develop cryopreservation methods for oocytes and embryos of Vietnamese native pigs”. This short-term study was to train Vietnamese counterpart on skills to undertake experiments to develop the most appropriate method of cryopreservation for embryos production.

Embryos were produced by in-vitro maturation, fertilization and culture of Landrace x Large white x Duroc oocytes collected from slaughterhouse-derived ovaries.

Two vitrification methods, the Cryotop and the microdrop method were compared for the cryopreservation of embryos at two different developmental stages (ie. on blastocyst stage embryos and zygotes). At the zygote stage, high survival rates were achieved by both methods; however, at the blastocyst stage Cryotop was more effective.

The Project will undertake the same experiments with Vietnamese native pigs in order to develop the most effective methods of cryopreservation (such as defining the optimum media for embryo production).

Van2 Van5

Some photos of the training course

Related Articles

20170928_112646

Introduction of some scientific results of the project at World...

Results on project’s in vitro fertilization using Ban frozen sperm experiments and vitrification of Ban oocytes experiments were introduced at the 4th World...
20170615_103330

First pure Ban blastocysts from project’s IVP system

In the framework of in vitro production of native pig embryo, the first experiment for fertilization of Ban oocytes with Ban sperms has been carried out with...
hayashi 3

Dr. Hayashi Visits Model Farms in Cao Son Commune, Da Bac District,...

It has been almost one year since the Project started to work with the model farms in Cao Son Commune, Da Bac District of Hoa Binh. Currently, there are 6 model...