Short-term Study in Japan about Vitrification Procedures for Embryos, 20 Feb – 18 March, 2017
One of the outputs of the Project is to “develop cryopreservation methods for oocytes and embryos of Vietnamese native pigs”. This short-term study was to train Vietnamese counterpart on skills to undertake experiments to develop the most appropriate method of cryopreservation for embryos production.
Embryos were produced by in-vitro maturation, fertilization and culture of Landrace x Large white x Duroc oocytes collected from slaughterhouse-derived ovaries.
Two vitrification methods, the Cryotop and the microdrop method were compared for the cryopreservation of embryos at two different developmental stages (ie. on blastocyst stage embryos and zygotes). At the zygote stage, high survival rates were achieved by both methods; however, at the blastocyst stage Cryotop was more effective.
The Project will undertake the same experiments with Vietnamese native pigs in order to develop the most effective methods of cryopreservation (such as defining the optimum media for embryo production).
Some photos of the training course